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placeholder. Metagenome

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posted on 2024-09-29, 06:32 authored by USDA-ARS-Beltsville Animal Biosciences and Biotechnology Laboratory (ABBL)
The aim of this study was to determine the effect of different 16S rRNA primers on taxonomic composition, diversity, and predicted function of chicken cecal microbial community. Cecal digesta collected from Ross 708 birds at 1, 3 and 5 weeks of age were used for bacterial DNA isolation. Eight different primer pairs targeting different variable regions of the 16S rRNA were employed. DNA sequences were analyzed using open source platform Qiime2 and Greengenes database for alpha and beta diversity and taxonomic composition. PICRUSt was used to determine the predicted function of bacterial communities.

History

Data contact name

BioProject Curation Staff

Data contact email

bioprojecthelp@ncbi.nlm.nih.gov

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2020-11-23

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

metagenomics; sequence analysis

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA680391

Preferred dataset citation

It is recommended to cite the accession numbers that are assigned to data submissions, e.g. the GenBank, WGS or SRA accession numbers. If individual BioProjects need to be referenced, state that "The data have been deposited with links to BioProject accession number PRJNA680391 in the NCBI BioProject database (https://www.ncbi.nlm.nih.gov/bioproject/)."

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    Keywords

    metagenome

    Licence

    Copyright Undetermined

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