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oyster hemolymph transcriptomes challenged with Perkinsus marinus and apoptosis modulators

dataset
posted on 2024-01-27, 00:20 authored by University of Rhode Island
Pooled eastern oyster hemolymph from a mixture of selectively bred families (VIMS) was challenged in triplicate with filtered sterile seawater (FSSW) (control), Perkinsus marinus (1:1 MOI with hemocyte concentration) for 1hr, P. marinus (1:1) for 1hr following a 3hr incubation with the IAP inhibitor GDC-0152, or P. marinus (1:1) for 1hr following a 1hr incubation with the caspase inhibitor Z-VAD-FMK. Samples were flash frozen with liquid nitrogen after 1hr of challenge and stored at -80C prior to RNA extraction. RNA was extracted from hemocyte samples using a Trizol protocol and sequenced at a depth of 15-20 million reads per sample (12 samples total).

Funding

National Institute of Food and Agriculture, 2019-67011-29553

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2021-10-06

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

sequence analysis

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA769054

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