bacterial 16S sequences from the guts of honey bees fed fungicide Targeted Locus (Loci)

The goal of this study was to determine whether the microbial communities present in honey bee guts were impacted by fungicide (Pristine). The fungicide was applied to loose pollen and the bees foraged on this pollen and brought it back to their hives. Bees were reared in the hives and were collected when they were performing nurse worker duties. The guts (not including the crop) were dissected, total DNA was extracted, and the 16S rRNA gene sequence was amplified from the total DNA. This DNA was then sequenced and analyzed to determine whether guts from nurse worker bees fed fungicide harbored a different microbiota than guts from nurse worker bees fed pollen without fungicide.