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Zea mays Transcriptome or Gene expression

dataset
posted on 2024-06-11, 05:07 authored by Maize Genetics, Corn Host Plant Resistance Research Unit (CHPRRU), USDA-ARS
The experiment consisted of a field design containing two maize inbred lines Va35 and Mp313E. Va35 has yellow kernels and is susceptible to kernel infection by Aspergillus flavus (A. flavus). Mp313E is a white dent maize inbred line and was released primarily as a source of resistance to kernel infection by A. flavus. The test ears were inoculated with Aspergillus flavus and collected two days after inoculation. Non-inoculated ears collected 16 days after pollination were also used as a control. The microarray experimental design was a randomized complete block design with three replications. One microarray slide was used for each field plot. Each slide contained the inoculated and non-inoculated sub-treatments. A second slide for one replication of each genotype contained a dye swap for the inoculation treatment and three subsamples (dots) within the slide represented each contig. Keywords: Direct comparison Overall design: Two-condition experiment, inoculated vs. uninoculated maize ears for Va35 and Mp313E. Biological replicates: 3 control, 3 inoculated, 2 dye-swap, independently grown and harvested. One replicate per array.

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2007-11-08

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

transcriptome; gene expression

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA103361

Preferred dataset citation

It is recommended to cite the accession numbers that are assigned to data submissions, e.g. the GenBank, WGS or SRA accession numbers. If individual BioProjects need to be referenced, state that "The data have been deposited with links to BioProject accession number PRJNA103361 in the NCBI BioProject database (https://www.ncbi.nlm.nih.gov/bioproject/)."

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