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Whole genome sequences of bacteria isolated from Oil fly (Helaeomyia petrolei) larvae

dataset
posted on 2024-05-29, 21:50 authored by Lisa DursoLisa Durso, Md ShamimuzzamanMd Shamimuzzaman, Brian Dillard, Kenneth Nickerson

Larvae from the petroleum oil fly, Helaeomyia petrolei, live in the asphaltene and polyaromatic hydrocarbon rich asphalt seeps of Rancho La Brea, Los Angeles, California. These larvae pass high amounts of viscous asphalt through their digestive system, and their gut microbiota is exposed to these extreme conditions. Environmental stress response mechanisms can co-select for antibiotic resistance, and in the current study we used 16S rRNA and genomic sequencing along with the Comprehensive Antibiotic Resistance Database (CARD) tools to characterize antibiotic resistance profiles from six bacteria previously isolated from the oil fly larval intestinal tract, linking phenotypic and genotypic resistance profiles. The isolates contain a core set of antibiotic resistance determinants along with determinants that are rarely found in these species. Comparing these oil fly isolates to the phenotypic prevalence data generated by the CARD Resistance Gene Identifier revealed sixteen instances where the oil fly bacteria appeared to carry a resistance not seen in related taxa in the database, suggesting a novel suite of resistance families in the oil fly isolates compared to other members of the same taxa. Results highlight the functional duality of genes that code for antibiotic resistance and expand our understanding of the ecological and evolutionary role of antibiotic resistance genes in environmental habitats.

Funding

USDA-ARS: 3042-12630-004-000D

History

Data contact name

Durso, Lisa M.

Data contact email

lisa.durso@usda.gov

Publisher

Ag Data Commons

Intended use

For use in peer reviewed publication: Novel antibiotic resistance profiles in bacteria isolated from Oil Fly larvae Helaeomyia petrolei living in the La Brea Tar Pits

Temporal Extent Start Date

2021-05-04

Temporal Extent End Date

2024-04-30

Theme

  • Non-geospatial

Geographic Coverage

{"type":"FeatureCollection","features":[{"geometry":{"type":"Point","coordinates":[-118.3491,34.0698]},"type":"Feature","properties":{}}]}

Geographic location - description

Bacteria isolated from oil fly larvae that lived in La Brea Tar Pits, Los Angeles, California. 34.0698° N, 118.3491° W

ISO Topic Category

  • biota

Ag Data Commons Group

  • ARS Culture Collection

National Agricultural Library Thesaurus terms

nucleotide sequences; oils; petroleum; asphaltenes; bitumen; California; intestinal microorganisms; stress response; antibiotic resistance; ribosomal RNA; sequence analysis; databases; bacteria; intestines; phenotype; antibiotic resistance genes; habitats; Diptera; insect larvae

OMB Bureau Code

  • 005:18 - Agricultural Research Service

OMB Program Code

  • 005:040 - National Research

ARS National Program Number

  • 212

ARIS Log Number

415634

Pending citation

  • Yes

Related material without URL

Durso, L.M., MD. Shamimuzzaman, B. Dillard, and K.W. Nickerson. Novel antibiotic resistance profiles in bacteria isolated from Oil Fly larvae Helaeomyia petrolei living in the La Brea Tar Pits.Submitted to Antonie van Leeuwenhoek (Journal).

Public Access Level

  • Public