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Using next generation sequencing to compare gene expression of wild type and Δcsp1 mutant of bacterial plant pathogen Xylella fastidiosa strain Stag's Leap

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posted on 2024-06-11, 06:44 authored by USDA-ARS

To investigate the role(s) of a cold shock protein homolog (Csp1) in plant pathogenic bacteria Xylella fastidiosa, we compared transcriptome profiles between wild type and a csp1 deletion mutant (Δcsp1) using long read Nanopore RNA sequencing. Overall design: Examined mRNA profiles of 7 day old wild type and Δcsp1 X. fastidiosa cells grown on PD3 agar plates under standard conditions (28°C) and at low temperature conditions (15°C)

History

Data contact name

BioProject Curation Staff

Data contact email

bioprojecthelp@ncbi.nlm.nih.gov

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2021-09-22

Theme

Non-geospatial

ISO Topic Category

biota

National Agricultural Library Thesaurus terms

transcriptome; gene expression

Pending citation

Public Access Level

Public

Accession Number

PRJNA765323

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It is recommended to cite the accession numbers that are assigned to data submissions, e.g. the GenBank, WGS or SRA accession numbers. If individual BioProjects need to be referenced, state that "The data have been deposited with links to BioProject accession number PRJNA765323 in the NCBI BioProject database (https://www.ncbi.nlm.nih.gov/bioproject/)."

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Keywords

Xylella fastidiosa subsp. fastidiosa eBacteria

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