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Transcriptomics of L. monocytogenes treated with sodium lactate

dataset
posted on 2024-06-11, 05:42 authored by USDA
L. monocytogenes F2365 strain was grown in Brain Heart Infusion (BHI) liquid broth overnight at 37°C with 200 rpm agitation. The stationary phased bacterial cells were diluted 1:5 in BHI liquid broth supplemented with 4% sodium lactate, grown at 15°C in static for 2 d. Bacterial cells without sodium lactate grown at the same condition (2 d) were used as controls. Bacterial RNA was isolated using RiboPure RNA Purification Kit (Thermo Fisher Scientific). rRNA was depleted from total RNA using Ribo-Zero Magnetic Kit (Illumina). For fragmentation and library preparation, Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) was used according to the manufacturer’s instruction. RNA and DNA quality and library quantification were performed using using a 2100 Bioanalyzer (Agilent Technologies). Sequencing was carried out using Ion Chef and Ion Torrent PGM instruments (Thermo Fisher Scientific).

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2017-04-11

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

transcriptome; gene expression

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA382528

Preferred dataset citation

It is recommended to cite the accession numbers that are assigned to data submissions, e.g. the GenBank, WGS or SRA accession numbers. If individual BioProjects need to be referenced, state that "The data have been deposited with links to BioProject accession number PRJNA382528 in the NCBI BioProject database (https://www.ncbi.nlm.nih.gov/bioproject/)."

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