Transcriptome sequencing of axillary buds of switchgrass Pvtb1 mutant and WT plant.

It was shown in the expression database that Pvtb1 genes in switchgrass expressed highly in axillary buds (Panicum virgatum v1.0, DOE-JGI, http://phytozome.jgi.doe.gov/). Thus, we extracted RNAs from axillary buds of tillers. The mutant 52-1 with highly enhanced tiller production and the WT plant (WT-1) generated from the same callus line from which the mutant 52-1 was obtained were chosen for mRNA-seq. RNA-seq analysis revealed a complex regulatory network potentially regulating tillering in switchgrass and provided some clues to the pathways of Pvtb1 genes.