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Transcriptome analysis of a nematode resistant and susceptible upland cotton line

dataset
posted on 2024-01-26, 23:46 authored by USDA-ARS
We applied the comparative transcriptomic approach to compare expression profiles of genes between RKN susceptible and resistance genotypes at an early stage of RKN development that coincides with the establishment of a feeding site and at the late stage of RKN development that coincides with RKN egg production. Sequencing of cDNA libraries produced over 315 million reads of which 240 million reads (76%) were mapped on to the Gossypium hirsutum genome. Overall design: Replicated set of cDNA libraries were constructed from M120 and C201 plants at 12, 30 DAI and untreated plants. All libraries were constructed using the NEBNext Ultra RNA library Prep Kit for Illumina (New England BioLabs Inc.) following the manufacturers protocol. The NEBNext Multiplex Oligos for Illumina (Index Primers Set 2, New England BioLabs Inc.) containing adaptors and primers were used to label libraries facilitating pooling.

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2019-08-21

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

transcriptome; gene expression

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA561377

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