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TnSeq mapping and BarSeq quantification of Ralstonia mutant fitness

dataset
posted on 2024-09-29, 06:18 authored by USDA-ARS, QB3 Berkeley Genomics
We created transposon insertion libraries in 3 plant pathogenic Ralstonia isolates using pKMW3, a randomly barcoded Mariner transposon vector. Mutant libraries were created in R. pseudosolanacearum GMI1000, R. solanacearum IBSBF1503, and R. syzygii PSI07. (Note: The genomes for several strains are still classified as R. solanacearum, but the names here reflect the current taxonomy). See PMID: 25968644 for plasmid reference, and TnSeq / BarSeq protocols.

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2020-04-28

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

sequence analysis

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA629015

Preferred dataset citation

It is recommended to cite the accession numbers that are assigned to data submissions, e.g. the GenBank, WGS or SRA accession numbers. If individual BioProjects need to be referenced, state that "The data have been deposited with links to BioProject accession number PRJNA629015 in the NCBI BioProject database (https://www.ncbi.nlm.nih.gov/bioproject/)."

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