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TnSeq mapping and BarSeq quantification of Ralstonia mutant fitness

dataset
posted on 2024-01-26, 23:54 authored by USDA-ARS, QB3 Berkeley Genomics
We created transposon insertion libraries in 3 plant pathogenic Ralstonia isolates using pKMW3, a randomly barcoded Mariner transposon vector. Mutant libraries were created in R. pseudosolanacearum GMI1000, R. solanacearum IBSBF1503, and R. syzygii PSI07. (Note: The genomes for several strains are still classified as R. solanacearum, but the names here reflect the current taxonomy). See PMID: 25968644 for plasmid reference, and TnSeq / BarSeq protocols.

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2020-04-28

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

sequence analysis

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA629015

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