The effect of food processing on fermentable oligosaccharides from dry beans in human colon and its microbiota

Gas production and bloating remain significant barriers to consumption of certain flatulogenic foods. The first objective of this study examines the role of Megasphaera elsdenii in gas production by the microbiome during fermentation of kidney beans and sweet potatoes: two foods commonly associated with gas production and bloating. The second objective determined raffinose degradation during fermentation among 21 microbiomes and associated raffinose degradation to gas production, short chain fatty acid production and microbiome composition to determine features related to gas production during degradation of raffinose. The approach used in this study are in vitro batch fermentation experiments using fecal microbiota from healthy human subjects.