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The beef cattle rumen microbiome composition is influenced by host genetics

Name: The beef cattle rumen microbiome composition is influenced by host genetics
Published: 2024-09-29T06:09:39+00:00
License: https://rightsstatements.org/vocab/UND/1.0/
Keywords: raw sequence reads

dataset

posted on 2024-09-29, 06:09 authored by University of Nebraska Lincoln

DNA was extracted from the rumen samples using the PowerMag Soil DNA isolation kit (MoBio Laboratories, Carlsbad, CA, USA) according to the manufacturer’s protocol with a few modifications as described by publication. The modified protocol also included adding RNase A to the lysis solution to ensure removal of RNA during DNA extraction. The isolated DNA was stored at -20°C until used for bacterial community analysis. The V4 region of the 16S rRNA gene was amplified and sequenced on the MiSeq platform.

Funding

1) USDA; 2) HMR: 2018-67015-27496; W2010

History

Data contact name

BioProject Curation Staff

Data contact email

bioprojecthelp@ncbi.nlm.nih.gov

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2019-07-03

Theme

Non-geospatial

ISO Topic Category

biota

National Agricultural Library Thesaurus terms

sequence analysis

Pending citation

Public Access Level

Public

Accession Number

PRJNA552591

Preferred dataset citation

It is recommended to cite the accession numbers that are assigned to data submissions, e.g. the GenBank, WGS or SRA accession numbers. If individual BioProjects need to be referenced, state that "The data have been deposited with links to BioProject accession number PRJNA552591 in the NCBI BioProject database (https://www.ncbi.nlm.nih.gov/bioproject/)."

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raw sequence reads

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