File(s) stored somewhere else
Please note: Linked content is NOT stored on Ag Data Commons and we can't guarantee its availability, quality, security or accept any liability.
The beef cattle rumen microbiome composition is influenced by host genetics
dataset
posted on 2024-06-11, 06:13 authored by University of Nebraska LincolnDNA was extracted from the rumen samples using the PowerMag Soil DNA isolation kit (MoBio Laboratories, Carlsbad, CA, USA) according to the manufacturer’s protocol with a few modifications as described by publication. The modified protocol also included adding RNase A to the lysis solution to ensure removal of RNA during DNA extraction. The isolated DNA was stored at -20°C until used for bacterial community analysis. The V4 region of the 16S rRNA gene was amplified and sequenced on the MiSeq platform.
Funding
U.S. Department of Agriculture, 2018-67015-27496; W2010
History
Data contact name
BioProject Curation StaffData contact email
bioprojecthelp@ncbi.nlm.nih.govPublisher
National Center for Biotechnology InformationTemporal Extent Start Date
2019-07-03Theme
- Non-geospatial
ISO Topic Category
- biota
National Agricultural Library Thesaurus terms
sequence analysisPending citation
- No
Public Access Level
- Public