posted on 2024-09-29, 05:33authored byIowa State University, Meat Animal Research Center, ARS, USDA, Clay Center, NE, USA
To finely annotate a newly assembled PacBio long-read based genome, multiple tissues from the same single cross-bred domestic pigs were sequenced by PacBio IsoSeq and Illumina RNA-seq. The "full-length, non-chimeric" IsoSeq reads were error corrected with the cleaned, merged and digitally normalized RNA-seq reads by combining the SMRT Analysis software with Proovread.
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