Survivorship and gut microbial community of Bombus vosnesenskii exposed to an herbicide, a fungicide, an insecticide, or the combination of all three chemicals
We investigated site-level differences in survival and microbiome structure of wild bumble bees exposed to multiple pesticides, both individually and in combination. We collected wild Bombus vosnesenskii foragers (N = 175) from an alpine meadow, a valley lake shoreline, and a suburban park and maintained them on a diet containing an herbicide (glyphosate), a fungicide (tebuconazole), an insecticide (imidacloprid), or a combination of these chemicals. The herbicide treatment was 30 ppm of glyphosate (Sigma-Aldrich, USA), the fungicide treatment was 30 ppb of tebuconazole (Sigma-Aldrich, USA), and the insecticide treatment was 30 ppb of imidacloprid (Sigma-Aldrich, USA). These chemicals were selected because they had the highest estimated use for each chemical class (insecticide, herbicide, fungicide) in our region (Washoe county, Nevada, (Wieben 2019)). These concentrations of imidacloprid and tebuconazole approximate the median of those detected in nectar (Zioga et al. 2020). The concentration we chose for glyphosate is within the range of field-realistic exposure from treated plants (Herbert et al. 2014, Thompson et al. 2022, Helander et al. 2023b) although is likely higher than bees would experience foraging from non-target plants (Zioga et al. 2023). We monitored survival daily for 20 days. Every 3 days, we replaced feeders with a clean feeder with 1000 mL of solution. We continued the experiment until bees died or at day 20 (whichever came first) at which point we weighed the bees, measured their intertegular (IT) span to estimate body size (Cane 1987) and dissected out the midgut and hindgut (hereafter ‘gut’) for DNA extractions and 16s sequencing (see below).
Funding
The impacts of multiple agricultural chemicals on the microbial ecology of pollination and implications for wild bumblebee health
National Institute of Food and Agriculture
Find out more...History
Data contact name
Tatarko, AnnaData contact email
atatarko09@gmail.comPublisher
Ag Data CommonsIntended use
ResearchTemporal Extent Start Date
2022-06-21Temporal Extent End Date
2022-07-05Frequency
- continual
Theme
- Non-geospatial
Geographic Coverage
{ "type": "FeatureCollection", "features": [ { "geometry": { "type": "Point", "coordinates": [ -119.919090, 39.301880 ] }, "type": "Feature", "properties": {} }, { "geometry": { "type": "Point", "coordinates": [ -119.7724167, 39.2362222 ] }, "type": "Feature", "properties": {} }, { "geometry": { "type": "Point", "coordinates": [ -119.8626944, 39.5010000 ] }, "type": "Feature", "properties": {} } ] }Geographic location - description
Washoe county, Nevada. Sites ranged from 14.58 km – 30.46 km apart and differed in elevation and degree of urbanization. Alpine Meadow (39.3018800°, -119.9190897°, elev: 2611.52, urbanization score: 1.93) Scrubland Shoreline (39.2362222°, -119.7724167°. elev: 1535.58, urbanization score: 0.92) Suburban Park (39.5010000°, -119.8626944°, elev: 1420.06, urbanization score: -2.85).ISO Topic Category
- environment
- biota
- farming
National Agricultural Library Thesaurus terms
survival rate; intestinal microorganisms; microbial communities; Bombus; microbiome; alpine meadows; lakes; shorelines; diet; glyphosate; tebuconazole; imidacloprid; pesticide application; Nevada; nectar; bees; foraging; body size; midgut; hindgut; DNA; agrochemicals; pollinatorsOMB Bureau Code
- 005:20 - National Institute of Food and Agriculture
Pending citation
- Yes
Related material without URL
Sequencing data is available on the NCBI Sequence Read Archive (SRA) BioProject ID number PRJNA1221307.Public Access Level
- Public