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Spatial transcriptomics study of Wooden Breast Myopathy in Broiler Chickens

dataset
posted on 2025-01-23, 16:48 authored by Behnam AbashtBehnam Abasht

This study aims to use spatial transcriptomics to characterize the cell-type-specific expression profile associated with the microscopic features observed in Wooden Breast myopathy. 1 cm3 muscle sample was dissected from the cranial part of the right pectoralis major muscle from three randomly sampled broiler chickens at 23 days post-hatch and processed with Visium Spatial Gene Expression kits (10X Genomics), followed by high-resolution imaging and sequencing on the Illumina Nextseq 2000 system. WB classification was based on histopathologic features identified. Sequence reads were aligned to the chicken reference genome (Galgal6) and mapped to histological images. Unsupervised K-means clustering and Seurat integrative analysis differentiated histologic features and their specific gene expression pattern, including lipid laden macrophages (LLM), unaffected myofibers, myositis and vasculature. In particular, LLM exhibited reprogramming of lipid metabolism with up-regulated lipid transporters and genes in peroxisome proliferator-activated receptors pathway, possibly through P. Moreover, overexpression of fatty acid binding protein 5 could enhance fatty acid uptake in adjacent veins. In myositis regions, increased expression of cathepsins may play a role in muscle homeostasis and repair by mediating lysosomal activity and apoptosis. A better knowledge of different cell-type interactions at early stages of WB is essential in developing a comprehensive understanding.

Funding

Targeting genomic regions identified through genome-wide association study of Wooden Breast

National Institute of Food and Agriculture

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History

Data contact name

Abasht, Behnam

Data contact email

abasht@udel.edu

Publisher

NCBI

Temporal Extent Start Date

2023-09-15

Theme

  • Non-geospatial

ISO Topic Category

  • farming

National Agricultural Library Thesaurus terms

transcriptomics; breasts; muscles; broiler chickens; gene expression; genomics; histology; gene expression regulation; macrophages; myositis; lipid metabolism; genes; peroxisome proliferator-activated receptors; fatty acid-binding proteins; fatty acids; cathepsins; homeostasis; apoptosis; poultry diseases

OMB Bureau Code

  • 005:20 - National Institute of Food and Agriculture

OMB Program Code

  • 005:040 - National Research

Pending citation

  • No

Public Access Level

  • Public