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Spatial and temporal transcriptional response to drought by Suillus pungens and Pinus muricata

dataset
posted on 2024-01-27, 00:20 authored by USDA-ARS
In this study, we used RNA sequencing to measure transcriptional changes in the fungus Suillus pungens (SP) exposed to experimental drought. We grew SP in symbiosis with Pinus muricata (PM) in a growth chamber where we manipulated both long-term (chronic) and short-term (acute) soil moisture and sequenced mRNA extracted from both ectomycorrhizal roots and extraradical mycelium (ERM). Our primary aims were to: (i) demonstrate the utility of RNA sequencing to quantify response to global change stressors for EMF, (ii) identify biological functions involved in drought response by comparing gene expression across moisture treatments and in different parts of EMF, and (iii) estimate the potential for fungal acclimation by comparing gene expression between chronic and acute drought stress treatments.

Funding

National Science Foundation, 1845544

U.S. Department of Energy, 502931

National Institutes of Health, S10OD018220

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2021-10-14

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

sequence analysis

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA771376

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