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Soybean Aphids per Plant Among Soybean Lines Containing Various Rag Genes

posted on 2024-02-15, 22:19 authored by Louis HeslerLouis Hesler, Eric A. Beckendorf

The soybean aphid (Aphis glycines) is an insect pest of cultivated soybeans (Glycine max). Several genes with resistance to A. glycines (i.e. Rag genes) have been identified in soybean. Virulent strains of soybean aphid are able to overcome the resistance and colonize soybeans having one or more Rag genes. It is important to classify virulent strains of soybean aphids in evaluating soybean lines in order to develop cultivars with durable resistance. The files presented here report the number of soybean aphids on soybean lines that differed in the Rag genes they contained. Two colonies of soybean aphid were tested.

Materials and Methods

Tests were conducted separately against the two soybean aphid colonies, which were maintained on soybean plants at North Central Agricultural Research Laboratory (NCARL), USDA-ARS, Brookings, South Dakota, USA, largely according to procedures described in Hesler and Tilmon (2018). The first colony was established from a single aphid collected near Volga, South Dakota, USA in 2016 and designated as ‘Volga16’ (Conzemius et al. 2019). It was reared on soybean cultivar ‘LD12R12-15805Ra’ (Rag1+Rag2 pyramid; University of Illinois, Urbana-Champaign, IL, USA).

A second colony designated ‘Accrue’ was derived from a colony originally established from a single first instar isolated from aphids collected at Urbana, IL, USA, and initially reared in Urbana (‘Urbana clone’; Hill et al. 2004). This colony was established as an avirulent soybean aphid colony (Hill et al. 2004). A series of sequential colonies from the initial colony was established, in order, at The Ohio State University, Wooster, OH, USA; Iowa State University, Ames, IA, USA; South Dakota State University, Brookings, SD, USA; and finally, in 2018 at NCARL. Although established as an ostensibly avirulent colony derived from the ‘Urbana clone’ colony, it was unexpectedly virulent against a known resistant accession, LD05R-16137 (containing Rag1), in initial screening tests.

Two separate no-choice tests were run for each soybean aphid colony. Each test consisted of seven soybean lines. Six had one or more Rag genes: 19APH18 (Rag1), 19APH25 (Rag2), 19INC (Rag3), 19APH29 (Rag4), 19APH30 (Rag6), 19APH09Rag12 (a Rag1+Rag2 pyramid); and ‘Titan,’ an aphid-susceptible soybean cultivar (Diers et al. 1999). Two-week-old, unifoliate-stage soybean plants growing in plastic pots (6 cm top diameter, 4 cm bottom diameter, 5.7 cm height) were each infested with 10 apterous adult soybean aphids and covered with a clear plastic, ventilated, cylindrical tube. After 20 days in an environmental chamber, the shoots of test plants were clipped at soil level, placed individually in sealable plastic bags, and stored in a freezer. Plants were removed over the next few days, and the aphids on them were counted. The data are contained in separate files—one for each of two soybean aphid colonies.

Resources in this dataset:

  • Resource Title: Number of Soybean Aphids Accrue Colony vs Rag Lines.

    File Name: Number of Soybean Aphids Accrue Colony vs Rag Lines.xlsx

    Resource Description: Number of Accrue colony soybean aphids per plant on various Rag soybean lines from no-choice laboratory tests.

  • Resource Title: Number of Soybean Aphids Volga16 Colony vs Rag Lines.

    File Name: Number of Soybean Aphids Volga16 Colony vs Rag Lines.xlsx


USDA-ARS: 3080-21220-006-00D


Data contact name

Hesler, Louis

Data contact email


Ag Data Commons

Intended use

To characterize the virulence of a soybean aphid colony based on its population response to soybean lines containing one or more Rag genes.

Use limitations

To understand the spectrum of virulence within a particular soybean aphid colony.

Temporal Extent Start Date


Temporal Extent End Date



  • Not specified

Geographic Coverage


ISO Topic Category

  • biota
  • farming

National Agricultural Library Thesaurus terms

Aphis glycines; soybeans; genes; insect pests; virulent strains; South Dakota; rearing; Illinois; instars; Ohio; Iowa; virulence; screening; imagos; plastics; shoots; soil; plastic bags; freezers; pest resistance

OMB Bureau Code

  • 005:18 - Agricultural Research Service

OMB Program Code

  • 005:040 - National Research

ARS National Program Number

  • 304

Pending citation

  • No

Public Access Level

  • Public

Preferred dataset citation

Hesler, Louis S.; Beckendorf, Eric A. (2020). Soybean Aphids per Plant Among Soybean Lines Containing Various Rag Genes. Ag Data Commons.