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Sequencing and Assembly for Two Bacteria Isolated from Wheat Straw: Thermoclostridium stercorarium subsp. RKWS-1 and Thermoanaerobacter sp. RKWS-2

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posted on 2024-01-27, 00:34 authored by North Carolina State University
Plant biomass residues were found to harbor dormant anaerobic, thermophilic bacteria. The goal of this study was to understand what types of thermophilic life might persist in mesophilic biomass samples and to investigate novel carbohydrate-active enzymes from these organisms. In this project, two bacteria were isolated from wheat straw after incubation in "Low-Carbon" LC medium and "Media for Thermophilic Clostridia" MTC medium at 60C. Specifically, a (hemi)cellulolytic, anaerobic, bacterium, Thermoclostridium stercorarium RKWS1, and a non-cellulolytic anaerobic bacterium, Thermoanaerobacter sp. RKWS2, were isolated and their genomes sequenced.

Funding

U.S. Department of Agriculture, NIFA 2018-67021-27716

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2022-10-03

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

genomics; sequence analysis; genome assembly

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA886731

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