Sequence of PCR amplicon created using bombicar primers for partial small ribosomal subunit of Nosema bombi

The primary purpose of the project was to determine the prevalence of Nosema bombi infections in the bumble bee communities of Northern Arizona. DNA was extracted from a Nosema bombi infected Bombus occidentalis male, to be used as a positive control for PCR runs to determine the Nosema bombi infection status of wild caught bumble bees. To be certain that the amplicon created using the PCR primer 'bombicar' (and DNA extracted from the positive control bumble bee) was Nosema bombi, the amplicon was sequenced. We are now submitting the sequence data we obtained from this amplicon.