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Schizosaccharomyces cryophilus OY26 WGS sequencing

dataset
posted on 2024-01-27, 05:02 authored by Broad Institute
Before the official species description of Schizosaccharomyces cryophilus OY26 this strain was known as Schizosaccharomyces sp. strain OY26. The sequenced stain is a type strain and has been deposited at NRRL, Agricultural Research Service Culture Collection as NRRL Y-48691, at the Centraal Bureau voor Schimmelcultures as CBS 11777 and at NBRC, NITE Biological Resource Center as NBRC 106824. This strain was isolated as a contaminant of the Schizosaccharomyces octosporus strain CBS 7191. Genomic DNA from the S. cryophilus strain was submitted to 454 Life Sciences for whole-genome sequencing. A titanium fragment library was generated from 5 mg genomic DNA and sequenced on a single 454 run using titanium chemistry. A 3K long-tag paired-end (LTPE) library was generated in parallel from the same amount of genomic DNA and sequenced on half of an FLX run. Approximately 1.3 million high-quality reads of 400 bp were generated from the titanium run and 700 000 reads were generated from the LTPE FLX run. Quality-filtered sequences from the wholegenome shotgun fragment library and LTPE library were assembled using the 454 Newbler Assembler.

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2009-11-12

Theme

  • Non-geospatial

ISO Topic Category

  • biota

Ag Data Commons Group

  • ARS Culture Collection

National Agricultural Library Thesaurus terms

genomics; sequence analysis; genome assembly

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA38373

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