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Sap Microbiome of Southern California

dataset
posted on 2024-06-11, 06:12 authored by UCR
Grapevine is a pillar of the California state economy and agricultural identity. The grapevine vascular system plays a key role by transporting nutrient, water, and signals throughout the plant. We hypothesized that grapevine host in its sap, microbes thathave a beneficial impact on plant health by protecting against pathogen attack and supporting key biological processes. The negative pressure, low oxygen and nutrient content of the xylem sap make it a unique and unexplored microbial environment. Toaddress this hypothesis, we chose a vineyard under high Pierce’s disease (PD). PD is caused by the xylem-dwelling pathogenic bacterium Xylella fastidiosa. We selected ten grapevines within this vineyard with different PD severity ratings and monitoredthem over 2 growing seasons. We sampled the vine’s sap at key phenological stages (bloom, veraison, and post-harvest) and usedan amplicon metagenomics approach to profile the bacterial (16s -V4) and fungal (ITS) communities of the sap.

Funding

U.S. Department of Agriculture, 233883

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2019-06-12

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

sequence analysis

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA548584

Preferred dataset citation

It is recommended to cite the accession numbers that are assigned to data submissions, e.g. the GenBank, WGS or SRA accession numbers. If individual BioProjects need to be referenced, state that "The data have been deposited with links to BioProject accession number PRJNA548584 in the NCBI BioProject database (https://www.ncbi.nlm.nih.gov/bioproject/)."

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