Rhizoctonia solani AG-3 Rhs1AP Genome sequencing and assembly

The heterokaryotic genome of a field isolate (Rhs1AP) of R. solani anastomosis group 3 (AG-3) sampled from an infected potato stem in Maine in 1988, was sequenced using the Sanger ABI 3730xl, 454 Biosciences Titanium GS-FLX, and Illumina technologies. Sanger sequencing involved a multiple shotgun library strategy with different insert sizes (4 and 10 kb plasmid, and 40 kb fosmid) to obtain 6X sequence coverage and linkage of contigs, followed by assembly and annotation. This data was used in conjunction with Illumina reads and 2.5 Titanium runs to provide additional 10X coverage and linkage. An optical restriction map of the fungal chromosomes has been developed and used to validate the genome assembly. The optical map suggests that the genome size is ~86 Mb, there are at least 26 chromosomes ranging in size from 1.8 to 5.9 Mb, and a significant amount of inter-chromosomal duplications.