RNA-Seq of two soybean near-isogenic lines differing in the Rag5 locus, following infestation by soybean aphid biotype 2

This study aimed to investigate genome-wide changes in gene expression of two soybean genotypes following infestation with soybean aphids. Two near isogenic lines carrying either the Rag5 allele for resistance or the rag5 allele for susceptibility were used for this study. After infestation by biotype 2 aphids, transcriptomic changes in soybean leaf tissues over a two-day period (total 4 time points) was studied using Illumina HiSeq and subsequent high-throughput sequencing analysis pipeline.