RAD sequences - A first inference of the phylogeography of the worldwide invader Xylosandrus compactus
dataset
posted on 2024-11-23, 22:14authored byINRAE
Native to Southeastern Asia, the ambrosia beetle Xylosandrus compactus is invasive worldwide. Its invasion is favoured by its cryptic lifestyle, symbiosis with a fungus that facilitates a broad range of host plants, and predominant sib-mating reproduction.We used COI and RAD sequencing to i) characterise the worldwide genetic structure of the species, ii) disentangle the origin(s) of the non-native populations on the three invaded continents and (iii) analyse the genetic diversity and pathways within each invaded region.We used the RADIS pipeline (Cruaud et al. 2016) to i) demultiplex individuals using process_radtags (Catchen et al. 2013), ii) homogenise read length and remove a few low-quality bases by trimming reads to 139 bp and iii) remove PCR duplicates using clone_filter (Catchen et al. 2013). The obtained sequences are available here.
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