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Pseudomonas sp. CES Genome sequencing and assembly

dataset
posted on 2024-02-06, 23:57 authored by USDA-ARS-Beltsville Sustainable Agricultural Systems Laboratory
The CES strain was isolated from soil at the University of Iowa Center for Biocatalysis and Bioprocessing using an enrichment procedure with caffeine as sole carbon and nitrogen source. Prior to isolation, coffee grounds had been amended to the soil for ten days. The soil was then collected and incubated in M9 minimum medium with .25 percent caffeine as described previously previously (Yu CL, Louie TM, Summers R, Kale Y, Gopishetty S, Subramanian M. J Bacteriol. 2009 Jul;191(14):4624-32. Epub 2009 May 15.) The strain was initially characterized as Pseudomonas sp. Strain CES (for Coffee Enriched Soil) based on 16S rRNA sequencing and FAME analysis. CES uses the N-demethylation pathway for caffeine breakdown.

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2020-05-05

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

genomics; sequence analysis; genome assembly

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA613486

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