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Profiling of gut gene expression in Cry1Ab-resistant and susceptible strains of Ostrinia nubilalis reveals possible adaptations of resistant larvae to transgenic Cry1Ab corn

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posted on 2024-01-27, 05:01 authored by USDA-ARS
We revealed a total of 398 gut genes differentially expressed (i.e., either up- or down-regulated genes with expression ratio ≥2.0) in S-strain larvae, while only 264 gut genes differentially expressed in R-strain larvae after fed transgenic corn leaves. Although the percentages of down-regulated genes among the total number of differentially expressed genes (199 genes or 50% in S-strain; 119 genes or 45% in R-strain) were similar between the R and S-strains, the expression ratios of down-regulated genes were much higher in S-strain than in R-strain. These results imply that R-strain larvae had an increased ability to deal with transgenic corn as compared with S-strain larvae. We also revealed that 17 and 9 significantly up- or down-regulated gut genes from S- and R-strain larvae, respectively, including serine proteases (trypsins and chymotrypsins) and aminopeptidases. Overall design: Trangenic corn induced gene expression was measured in O. nubilalis resistant and susceptible strains' gut at 6-hrs after transgenic corn feeding. Three independent experiments were performed using three insect gut samples ,and each sample was from five insects' gut pooled.

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2016-09-27

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

transcriptome; gene expression

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA344650

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