posted on 2024-06-11, 05:54authored byUniversity of Florida
Pigs are the only common livestock species known to express CD1d-restricted invariant natural killer T (iNKT) cells. We exploited that porcine iNKT cells can be detected using a mouse CD1d-tetramer reagent loaded with the prototypic iNKT cell ligand, α-galactosylceramide to characterize the T cell receptor (TCR) usage of iNKT cells compared to conventional αβ T cells (Tconv). Using a TCR sequencing approach that does not require prior knowledge of any V sequences, we characterized the pig iNKT cell and Tconv TCR repertoire.
Funding
National Institute of Food and Agriculture, 2016-09448
National Institute of Child Health & Human Development, R01 HD092286
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