Porcine Amygdala RNA-Sequencing

Prebreeding anestrus is a major reason for reproductive failure in gilts. The two types of prebreeding anestrus are delay in attaining puberty (prepubertal, PP) and silent estrus (behavioral anestrus, BA). Differential gene expression in the amygdala was analyzed in three comparisons; 1) PP cases and cyclic controls at follicular phase of estrous cycle, 2) BA cases and cyclic controls at luteal phase of estrous cycle, and 3) gilts at different stages of the ovarian cycle (cyclic gilts at follicular phase and luteal phase of estrous cycle) to understand gene expression profiles associated with different pubertal phenotypes and how the steroid hormone milieu in different stages of the estrous cycle affects gene expression in this key brain region.