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PRRSV-2 infection chains in vaccinated and non-immunized pigs

dataset
posted on 2025-04-02, 02:52 authored by University of Minnesota
This study examines the impact of modified live virus (MLV) vaccination on PRRSV-2 evolution using sequential pig-to-pig infection chains. Weaned pigs were divided into vaccinated (two vaccine strains) and non-immunized groups and challenged with PRRSV-2 sub-lineage 1A (1-7-4). Virus transmission continued for ~84 days across six batches, with serum samples from two days/batch with lowest Ct sequenced via the GridION platform (Oxford Nanopore Technologies, UK). Vaccinated pigs exhibited over twice the genetic divergence from the inoculum compared to controls. Deep sequencing revealed faster quasispecies shifts in vaccinated pigs, leading to more homogeneous populations. However, vaccinated pigs had significantly lower viral loads, suggesting fitness constraints on highly diverging variants. These findings demonstrate MLV vaccination drives PRRSV-2 evolution, emphasizing the need for ongoing genomic surveillance.

Funding

USDA-NIFA: 2019-67015-29918

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2025-02-15

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

sequence analysis

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA1224032

Preferred dataset citation

It is recommended to cite the accession numbers that are assigned to data submissions, e.g. the GenBank, WGS or SRA accession numbers. If individual BioProjects need to be referenced, state that "The data have been deposited with links to BioProject accession number PRJNA1224032 in the NCBI BioProject database (https://www.ncbi.nlm.nih.gov/bioproject/)."

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