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Overexpression of SLIM1 transcription factor accelerates vegetative development in Arabidopsis thaliana

dataset
posted on 2024-06-11, 07:17 authored by Cereal Crops Research Unit, USDA-ARS
SLIM1 has a well established role in regulating transcriptional responses to sulfur deficiency in Arabidopsis thaliana. In order to investigate the impact of SLIM1 expression under sufficient nutrient conditions, we generated 35S::SLIM1 over-expression lines. SLIM1OX plants were found to have larger rosette area, bolt earlier, and enter developmental senescence earlier than Col-0 and slim1KO (slim1-cr) plants. RNA-seq followed by differential expression analysis was performed on rosette tissue at three timepoints. Overall design: Gene expression profiling analysis was performed with RNA-seq data from Arabidopsis thaliana rosettes to investigate the function of SLIM1/EIL3 (AT1G73730) in sufficient nutrient conditions. 5 geneotypes were included in the study: Col-0, slim1KO (slim1-cr), pH7WG (EV), and two independent 35S::SLIM1 OX lines. Individual rosettes of each genotype were harvested at three timepoints: 30 days after sowing, 37 days after sowing, and 44 days after sowing.

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2023-09-22

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

transcriptome; gene expression

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA1020153

Preferred dataset citation

It is recommended to cite the accession numbers that are assigned to data submissions, e.g. the GenBank, WGS or SRA accession numbers. If individual BioProjects need to be referenced, state that "The data have been deposited with links to BioProject accession number PRJNA1020153 in the NCBI BioProject database (https://www.ncbi.nlm.nih.gov/bioproject/)."

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