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Oncorhynchus mykiss isolate:Whale Rock Male DH Line Genome sequencing and assembly

dataset
posted on 2024-09-29, 06:57 authored by USDA/ARS
Currently, we are working towards generating a rainbow trout pan-genome reference that will better represent the genetic diversity in this species using de-novo chromosome level assemblies. Previously we have submitted the current reference genome from the Arlee line (USDA_OmykA_1.1; GCA_013265735.3) and an improved assembly for the Swanson line (Omyk_2.0; GCA_025558465.1). The Swanson YY male line was originated from a semi-domesticated resident fish from the Kenai Peninsula in Alaska with a haploid number of 29 chromosomes. The Arlee doubled haploid YY male line has a different genetic background from the Swanson line. It was originated from a well-known domesticated hatchery strain that was originally collected from the northern California coast. It has a haploid number of 32 chromosomes. The three Swanson chromosomes that are being divided to two acrocentric chromosomes are Omy04, 14 and 25. The Whale Rock line we used for this de-novo genome assembly is also a YY male. Similar to the Arlee line, it has 2N=64 chromosomes and it was originated from the Central California Coast. The one known major difference is that it was originated from a wild fish from a landlocked steelhead population. The de-novo chromosome level assembly of the rainbow trout WRM line genome has helped correcting minor errors in the previous genome assembly and will aid in better characterization of structural genome variants in rainbow trout and other salmonids and in the pan-gene annotation of multiple rainbow trout and salmonids genomes.

Funding

USDA: 8082-31000-013-000-D

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2023-04-07

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

genomics; sequence analysis; genome

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA928430

Preferred dataset citation

It is recommended to cite the accession numbers that are assigned to data submissions, e.g. the GenBank, WGS or SRA accession numbers. If individual BioProjects need to be referenced, state that "The data have been deposited with links to BioProject accession number PRJNA928430 in the NCBI BioProject database (https://www.ncbi.nlm.nih.gov/bioproject/)."

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