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Next Generation Amplicon Sequencing Improves Detection of Blastocystis Mixed Subtype Infections

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posted on 2024-01-26, 23:29 authored by United States Department of Agriculture
Next generation sequencing (NGS) was performed on 75 fecal samples from cattle to detect and characterize Blastocystis subtypes and investigate intra-host Blastocystis diversity. Blastocystis-specific primers were used to amplify the SSU rRNA gene. Sequences from NGS were compared to Sanger sequences and the results showed the NGS method is more sensitive and is able to identify mixed subtypes within single samples.

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2018-11-29

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

sequence analysis

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA507584

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