- No file added yet -

Next Generation Amplicon Sequencing Improves Detection of Blastocystis Mixed Subtype Infections

Name: Next Generation Amplicon Sequencing Improves Detection of Blastocystis Mixed Subtype Infections
Published: 2024-06-11T05:59:04+00:00
License: https://rightsstatements.org/vocab/UND/1.0/
Keywords: raw sequence reads

dataset

posted on 2024-06-11, 05:59 authored by United States Department of Agriculture

Next generation sequencing (NGS) was performed on 75 fecal samples from cattle to detect and characterize Blastocystis subtypes and investigate intra-host Blastocystis diversity. Blastocystis-specific primers were used to amplify the SSU rRNA gene. Sequences from NGS were compared to Sanger sequences and the results showed the NGS method is more sensitive and is able to identify mixed subtypes within single samples.

History

Data contact name

BioProject Curation Staff

Data contact email

bioprojecthelp@ncbi.nlm.nih.gov

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2018-11-29

Theme

Non-geospatial

ISO Topic Category

biota

National Agricultural Library Thesaurus terms

sequence analysis

Pending citation

Public Access Level

Public

Accession Number

PRJNA507584

Preferred dataset citation

It is recommended to cite the accession numbers that are assigned to data submissions, e.g. the GenBank, WGS or SRA accession numbers. If individual BioProjects need to be referenced, state that "The data have been deposited with links to BioProject accession number PRJNA507584 in the NCBI BioProject database (https://www.ncbi.nlm.nih.gov/bioproject/)."

Usage metrics

Keywords

raw sequence reads

Licence

Exports

RefWorks

BibTeX

Ref. manager

Endnote

DataCite

NLM