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Microbiome standard and non-DNA template controls

dataset
posted on 2024-01-26, 23:50 authored by USDA
ZymoBIOMICS Microbial Community Standard (Cat# D6300) was obtained from Zymo Research (Irvine, CA, USA) as the microbiome standard (positive controls). In addition, nuclease free water was used as a non-DNA template (negative) controls (NDT). The standard consists of 3 Gram-negative and 5 Gram-positive bacteria and 2 yeasts with a defined composition. Both NDT and the standard were processed along with experimental samples following the same protocol and parameters to validate the entire workflow, from total DNA extraction, sequencing, and data analysis.

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2020-01-16

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

sequence analysis

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA601657

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