Ag Data Commons
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Microbial community structure is affected by cropping sequences and poultry litter under long-term no-tillage

posted on 2024-02-09, 17:42 authored by Amanda AshworthAmanda Ashworth

Soil microorganisms play essential roles in soil organic matter dynamics and nutrient cycling in agroecosystems and have been used as soil quality indicators. The response of soil microbial communities to land management is complex and the long-term impacts of cropping systems on soil microbes is largely unknown. Therefore, changes in soil bacterial community composition were assessed in response to cropping sequences and bio-covers at long-term no-tillage sites. Main effects of four different cropping sequences of corn (Zea mays L.), cotton (Gossypium hirsutum L.), and soybean (Glycine max L.) were rotated in four year phases for 12-yrs at two Tennessee Research and Education Centers in a randomized complete block design with split-block treatments of four winter bio-covers: hairy vetch (Vicia villosa L.), wheat (Triticum aestivum L.), poultry litter, and a fallow control. Using Illumina high-throughput sequencing of 16S rRNA genes, bacterial community composition was determined. Composition, diversity, and relative abundance of specific taxa were correlated per cropping system, bio-cover, and their interaction. We found that i) richness and diversity varied temporally and spatially, coinciding with soil carbon, pH, nutrient levels, and climatic variability; ii) community composition varied by cropping system, with continuous corn, soybean, and the corn-soybean rotation presenting a hybrid of the continuous corn and soybean communities; however, continuous cotton resulted in the most varied assemblage; iii) bio-covers asserted the greatest influence on microbial communities; specifically poultry litter treatments differed from cover crops (all of which received inorganic-N). Consequently, microbial diversity was greatest under nutrient rich bio-covers (poultry litter) and high residue producing, less pesticide-intensive cropping sequences (soybean and corn compared to cotton), suggesting a more dynamic soil ecology under these no-till cropping systems. This suggests that nutrient management (inorganic fertilizers vs. animal manure) and greater crop rotations (within 4-yr phases) may directly drive phylogenetic community structure and subsequent ecosystem services across agricultural landscapes.

Resources in this dataset:

  • Resource Title: Microbial diversity based on crop rotation, cover crop, and poultry litter after 14-years of managment .

    File Name: Ashworth microbial data by PLOT.xlsx

  • Resource Title: Microbial diversity based on crop rotation, cover crop, and poultry litter after 14-years of managment.

    File Name: Ashworth microbial data by PLOT.csv

    Resource Description: CSV version of the data

  • Resource Title: Data Dictionary.

    File Name: Ashworth-PLOT-Jul2019-data-dictionary.csv

    Resource Description: Defines variables, data type, accepted values, size, if required, etc. for the tabular data.




Data contact name

Ashworth, Amanda


Ag Data Commons


  • Not specified

ISO Topic Category

  • biota
  • farming

National Agricultural Library Thesaurus terms

soil organic matter; biogeochemical cycles; agroecosystems; soil quality; land management; soil bacteria; community structure; cropping sequence; no-tillage; corn; Zea mays; cotton; Gossypium hirsutum; soybeans; Glycine max; Tennessee; education; winter; Vicia villosa; wheat; Triticum aestivum; poultry manure; fallow; high-throughput nucleotide sequencing; ribosomal RNA; genes; bacterial communities; soil; carbon; pH; nutrient content; hybrids; cover crops; soil ecology; nutrient management; mineral fertilizers; crop rotation; phylogeny; ecosystem services; agricultural land

OMB Bureau Code

  • 005:18 - Agricultural Research Service

OMB Program Code

  • 005:040 - National Research

ARS National Program Number

  • 212

Primary article PubAg Handle

Pending citation

  • No

Public Access Level

  • Public

Preferred dataset citation

Ashworth, Amanda (2019). Microbial community structure is affected by cropping sequences and poultry litter under long-term no-tillage. Ag Data Commons.

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