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Metagenomic testing of a historic fungal mycelial mat

dataset
posted on 2024-01-27, 00:11 authored by University of York
Shotgun sequencing was conducted on a historic Tlingit wall pocket, a work of art used as a receptacle for household items and designed to hang on the wall. The wall pocket was made in 1903 at Ketchikan, Alaska. Microscopy indicates the wall pocket was crafted from a fungal mycelial mat, likely produced by Fomitopsis officinalis (Laricifomes officinalis). DNA sequencing was conducted on the wall pocket, a lysis buffer used in cleaning the specimen (referred to as predigestion), and an extraction control (blank). Neither shotgun sequencing nor internal transcribed spacer (ITS) testing led to a taxonomic identification of the wall pocket. According to requirements of the project, all sequencing reads which map to the human reference genome have been removed.

Funding

U.S. Department of Agriculture, MIN-22-081

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2021-01-10

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

sequence analysis

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA691059

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