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Metagenomic amplicon sequencing of sarcocystis species in US beef

dataset
posted on 2024-01-27, 00:19 authored by USDA-ARS
A new amplicon sequencing protocol was developed to identify the co-infecting species of Sarcocystis in meat samples. This method targets a variable region of the 18S ribosomal DNA and uses metagenomic techniques to distinguish among coinfecting species. 47 samples from beef that were suspected of having eosinophilic myositis were subjected to the protocol revealing multiple Sarcocystis species in some samples including the zoonotic parasite Sarcocystis hominis. This method could be used to screen beef samples for food safety purposes.

Funding

U.S. Department of Agriculture, 8042-32000-113-00D

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2021-09-28

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

sequence analysis

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA767047

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