Mapping of bs5 and bs6 resistances against bacterial spot of pepper

Two recessive resistances, bs5 and bs6, confer non-race-specific resistance against bacterial spot of pepper caused by Xanthomonas euvesicatoria. Mapping of bs5 and bs6 will facilitate their use in breeding programs through marker-assisted selection and is also a crucial step towards understanding their mechanism of resistance. We used genotyping-by-sequencing approach to initially map the position of the two resistances. Segregant population for bs5 and bs6 were developed by crossing susceptible Early CalWonder (ECW) with near-isogenic lines ECW50R (bs5 introgression) or ECW60R (bs6 introgression). Genomic DNA was extracted from lyophilized young foliar tissues. ApeKI GBS library was constructed using previously published protocol (Elshire et al. 2011) and sequenced on one lane (single end, 101 bp) of an Illumina HiSeq 2500.