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Leaf endomicrobiomes of early and late planted sorghum seedlings

dataset
posted on 2024-01-27, 00:40 authored by South Dakota State University
Leaf fungal and bacterial communities from field-grown sorghum accessions (KS and SD) were characterized in response to chilling and non-chilling temperatures using amplicon sequencing. Bacterial primers 799F and 1115R were used to amplify 16S V5-V6. Fungal primers ITS1f and ITS2 were used to amplify ITS1. Locus-specific PCR was followed by index PCR with Illumina Nextera Kit v2 sets A, B, C, D. After PCR clean-up, amplicons were pooled equimolarly and sequenced on an Illumina MiSeq 2 x 300 at South Dakota State University Genomics Sequencing Center.

Funding

U.S. Department of Agriculture, 1014561

National Science Foundation, 1849206

Kansas Agriculture Experimental Station, 21-259-J

National Science Foundation, IOS-1350189

U.S. Department of Agriculture, SD00H697-20

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2023-03-29

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

sequence analysis

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA950075

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