Leaf endomicrobiomes of early and late planted sorghum seedlings

Leaf fungal and bacterial communities from field-grown sorghum accessions (KS and SD) were characterized in response to chilling and non-chilling temperatures using amplicon sequencing. Bacterial primers 799F and 1115R were used to amplify 16S V5-V6. Fungal primers ITS1f and ITS2 were used to amplify ITS1. Locus-specific PCR was followed by index PCR with Illumina Nextera Kit v2 sets A, B, C, D. After PCR clean-up, amplicons were pooled equimolarly and sequenced on an Illumina MiSeq 2 x 300 at South Dakota State University Genomics Sequencing Center.