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Leaf endomicrobiomes of early and late planted sorghum seedlings

dataset
posted on 2024-11-23, 22:24 authored by South Dakota State University
Leaf fungal and bacterial communities from field-grown sorghum accessions (KS and SD) were characterized in response to chilling and non-chilling temperatures using amplicon sequencing. Bacterial primers 799F and 1115R were used to amplify 16S V5-V6. Fungal primers ITS1f and ITS2 were used to amplify ITS1. Locus-specific PCR was followed by index PCR with Illumina Nextera Kit v2 sets A, B, C, D. After PCR clean-up, amplicons were pooled equimolarly and sequenced on an Illumina MiSeq 2 x 300 at South Dakota State University Genomics Sequencing Center.

Funding

USDA: SD00H697-20

NSF: IOS-1350189

NSF: 1849206

USDA: 1014561

KS-AES: 21-259-J

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2023-03-29

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

sequence analysis

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA950075

Preferred dataset citation

It is recommended to cite the accession numbers that are assigned to data submissions, e.g. the GenBank, WGS or SRA accession numbers. If individual BioProjects need to be referenced, state that "The data have been deposited with links to BioProject accession number PRJNA950075 in the NCBI BioProject database (https://www.ncbi.nlm.nih.gov/bioproject/)."

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