Ag Data Commons
Browse

Largemouth bass (Micropterus nigicans) gut microbiota in response to immersion vaccination.

dataset
posted on 2025-04-26, 03:58 authored by Auburn University
Recent data has indicated increased largemouth bass (LMB; Micropterus nigricans) production as culturists aim to capitalize on the high market value. As one of the leading causes of mortality in warmwater culture, motile Aeromonas septicemia (MAS; including Aeromonas hydrophila and Aeromonas veronii) poses a significant threat to production facilities. Three experimental vaccination periods of 0-, 2-, and 4-months were studied to determine the optimal timeframe for vaccinating LMB fingerlings. At time 0 fish were 5 g. For each experimental time point, formalin-killed cultures of A. veronii (ARS-LMB-32-2018) and A. hydrophila (ARS-LMB-2022-09) were administered via a 30-minute static immersion bath. The LMB were monitored for 30 days post-vaccination (DPV) to allow the specific immune response to be generated. Thereafter, distal intestines were sampled at 0-, 15-, and 30 DPV to characterize microbial gut communities. At 30 DPV, LMB were challenged using an intraperitoneal injection or a standardized fin-clip methodology and subjected to a 1 h static bath at 2x107 CFU mL-1. Cumulative percent mortality was monitored incrementally through the 7-day challenge duration. Alpha- and beta-diversity indices also showed no negative impact on microbial communities between vaccine treatments, while days post-vaccination showed changing communities within sham and Aeromonas spp. vaccinations. With this long-term study, we aimed to improve the understanding of LMB fingerling response to formalin-killed immersion bath vaccinations of A. veronii & A. hydrophila and the potential for mortality reduction.

Funding

USDA-NIFA: 2022-06028

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2025-04-17

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

sequence analysis

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA1252284

Preferred dataset citation

It is recommended to cite the accession numbers that are assigned to data submissions, e.g. the GenBank, WGS or SRA accession numbers. If individual BioProjects need to be referenced, state that "The data have been deposited with links to BioProject accession number PRJNA1252284 in the NCBI BioProject database (https://www.ncbi.nlm.nih.gov/bioproject/)."

Usage metrics

    Categories

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC