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Iron regulation in P. syringae DC3000

posted on 2024-06-11, 05:08 authored by Plant Microbe Interaction Research Unit, USDA-ARS
DC3000 cultures were grown under highly controlled conditions and after the addition of iron citrate or sodium citrate to the media. In the cultures supplemented with iron, we found that cell-associated iron increased rapidly while culture densities were not significantly different over 4 hours when compared to cultures with sodium citrate added. Microarray analysis of samples taken from before and after the addition of either sodium citrate or iron citrate identified 386 differentially regulated genes with high statistical confidence. Differentially regulated genes were clustered based on expression patterns observed between comparison of samples taken at different time points and with different supplements. This analysis grouped genes associated with the same regulatory motifs and/or had similar putative or known function. Keywords: iron response, environmental signal, time course Overall design: 5 biological replicates, 3 timepoint: 0h, 0.5h, 4h after addition of iron citrate or sodium citrate to increase final concentration by 50uM


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National Center for Biotechnology Information

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  • Non-geospatial

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  • biota

National Agricultural Library Thesaurus terms

transcriptome; gene expression

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  • Public

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It is recommended to cite the accession numbers that are assigned to data submissions, e.g. the GenBank, WGS or SRA accession numbers. If individual BioProjects need to be referenced, state that "The data have been deposited with links to BioProject accession number PRJNA110097 in the NCBI BioProject database ("

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