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In vivo assembly of sorgoleone biosynthetic pathway

dataset
posted on 2024-06-11, 06:30 authored by USDA, ARS
Sorgoleone, a hydrophobic compound exuded from root hair cells of Sorghum spp., accounts for much of the allelopathic activity. The enzymes involved in the biosynthesis of this compound have been identified and functionally characterized. To explore the possibility of producing sorgoleone in other species, a multi-gene DNA construct was prepared for the expression of genes required for sorgoleone biosynthesis in planta and deployed in Nicotiana benthamiana leaf tissues via Agrobacterium-mediated transient expression. The production of sorgoleone observed in agroinfiltrated leaves as detected by GC/MS resulted in the formation of necrotic lesions, indicating that the compound possesses significant phytotoxicity to these tissues. RNA-sequencing profiling revealed significant changes in gene expression in the host plant during the formation of sorgoleone-induced necrotic lesions.

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2020-09-01

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

sequence analysis

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA660791

Preferred dataset citation

It is recommended to cite the accession numbers that are assigned to data submissions, e.g. the GenBank, WGS or SRA accession numbers. If individual BioProjects need to be referenced, state that "The data have been deposited with links to BioProject accession number PRJNA660791 in the NCBI BioProject database (https://www.ncbi.nlm.nih.gov/bioproject/)."

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