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Immunoenhancing effects of Montanide adjuvant on recombinant coccidia antigen vaccination against Eimeria spp. and comparative microarray analysis of intestinal lymphocytes

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posted on 2024-06-11, 05:11 authored by USDA, ARS

This study utilized comparative global gene expression microarray analysis to evaluate the effects of two different Montanide adjuvants, ISA70 and ISA71, in promoting chicken protective immunity following vaccination with profilin, an actin-regulatory protein that is expressed by multiple Eimeria species. Overall design: Three-condition experiment with reference hybridization design, 1) ISA70 plus profilin immunized chickens vs. profilin alone and 2) ISA71 plus profilin vs. profilin alone, 2 biological replicates with dye-switching, total 4 replicates for each adjuvants

History

Data contact name

BioProject Curation Staff

Data contact email

bioprojecthelp@ncbi.nlm.nih.gov

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2012-09-10

Theme

Non-geospatial

ISO Topic Category

biota

National Agricultural Library Thesaurus terms

transcriptome; gene expression

Pending citation

Public Access Level

Public

Accession Number

PRJNA174796

Preferred dataset citation

It is recommended to cite the accession numbers that are assigned to data submissions, e.g. the GenBank, WGS or SRA accession numbers. If individual BioProjects need to be referenced, state that "The data have been deposited with links to BioProject accession number PRJNA174796 in the NCBI BioProject database (https://www.ncbi.nlm.nih.gov/bioproject/)."

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Keywords

Gallus gallus eEukaryotes

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