Image data of growth of Valencia sweet orange nonembryogenic cells on predicted points from a 5-factor response surface design

The data are images of tissue cultures of Valencia sweet orange nonembryogenic callus cells taken in 2006 at the U.S. Horticultural Research Laboratory, Ft. Pierce, Florida, USA to photo document treatments from an experiment designed to determine how well a model developed from a 5-factor response surface methodology (RSM) design predicted callus growth. Growth predictions for thirteen formulations are listed in the spreadsheet file 5 factor RSM Design_Predicted.xlsx. Six culture dishes were used to estimate growth for each formulation. A culture dish representative of the growth of each formulation was photographed. Each image is named to match the formulation. For example, the jpg image labeled 5-factor RSM_MS prediction 21.JPG is an image of the callus grown on MS formulation #21. This dataset includes 15 files – 14 image files and 1 Excel spreadsheet. Images were captured in JPEG (EXIF 2.2) format with a Nikon Coolpix 5400 digital camera equipped with a 1/1.8” (7.2 x 5.3 mm) CCD sensor at a resolution of 2592 x 1944 pixels. Each image is of a single culture plate with the top lid removed and photographed under cool white, fluorescent lighting.

The experimental setup for the 5-factor response surface design is described in - Niedz, R. P. and T. J. Evens (2007). "Regulating plant tissue growth by mineral nutrition." In Vitro Cellular & Developmental Biology - Plant 43(4): 370-381.