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Identification of new full length Arabidopsis genes - pilot arrays
dataset
posted on 2024-06-11, 06:53 authored by Plant Gene Expression Center, University of California, Berkeley / USDAIdentification of new and unpredicted full length Arabidopsis genes. Examination of cRNA prepared from Arabidopsis thaliana ecotype Columbia light grown 7-day old seedlings, light grown 7-day old seedlings treated at 4 degrees C for 24hrs, and etiolated 7-day old seedlings using pilot genome tiling arrays. Total RNA was isolated from seedlings with the TRIzol reagent procedure of Invitrogen, then poly(A)+ RNA was purified with Qiagen oligotex. One microgram poly(A)+ RNA was converted into ds-cDNA using T7-oligo(dT) primers. Biotin-labeled cRNA was generated by in vitro transcription reactions using ENZO labeling kit, fragmented and then 20 micrograms of fragmented cRNA were hybridized to the arrays according to Affymetrix instructions. Keywords: other
History
Data contact name
BioProject Curation StaffData contact email
bioprojecthelp@ncbi.nlm.nih.govPublisher
National Center for Biotechnology InformationTemporal Extent Start Date
2003-10-29Theme
- Non-geospatial
ISO Topic Category
- biota
National Agricultural Library Thesaurus terms
transcriptome; gene expressionPending citation
- No
Public Access Level
- Public