<p dir="ltr">Conjugation efficiency of <i>Salmonella</i> Genomic Island 4 (SGI-4) from donor <i>Salmonella enterica</i> serovar I 4,[5],12:i:- or a plasmid containing<i> oriT</i><sub>SGI-4</sub> to recipient strain <i>Salmonella enterica</i> serovar Typhimurium<i>.</i> The <i>oriT</i><sub>SGI-4</sub> nucleotide sequences are located in the region that spans nucleotides 4704600-4706000 of the <i>Salmonella enterica</i> serovar I 4,[5],12:i:- strain USDA15WA-1 genome (<a href="https://www.ncbi.nlm.nih.gov/nuccore/CP040686" target="_blank">CP040686</a>). Conjugation efficiency was calculated by dividing the colony forming units (CFU)/mL of transconjugants by the CFU/mL of donors. A transconjugant is a bacterial recipient that received DNA from the donor strain due to horizontal gene transfer.</p>