Genomes & Annotations Data: Genome Resources for the Ex-type of Phytophthora citricola, and well-authenticated isolates of P. hibernalis, P. nicotianae and P. syringae

Phytophthora is one of the most important genera of plant pathogens. In order to build robust molecular identification systems, it is very important to have information from well-authenticated specimens and in preference the Ex-type specimens. The reference genomes of well-authenticated specimens form a critical foundation for genetics, biological research, and diagnostic applications. In this study, we describe four draft Phytophthora genomes resources for the Ex-type of P. citricola BL34 (P0716 WPC) (118 contigs for 50 Mb), and well-authenticated specimens of P. syringae BL57G (P10330 WPC) (591 contigs for 75 Mb), P. hibernalis BL41G (P3822 WPC) (404 contigs for 84 Mb), and P. nicotianae BL162 (P6303 WPC) (3984 contigs for 108 Mb) generated with MinION long-read High-Throughput Sequencing (HTS) technology (Oxford Nanopore Technologies, ONT). Using these draft reads we assembled high coverage genomes of P. citricola with 291X coverage and 16,662 annotated genes; P. nicotianae with 205X coverage and 29,271 annotated genes; P. syringae with 76X coverage and 23,331 annotated genes, and P. hibernalis with 42X coverage and 21,762 annotated genes. We anticipate that these draft genomes will be helpful for various basic and applied research including diagnostics to protect US agriculture.

Subodh K. Srivastava (1, 2), Leandra M. Knight (1, 2), Mark Nakhla (1) and Z. Gloria Abad (1) 1 - USDA APHIS PPQ, S&T, Beltsville Laboratory, Beltsville, MD, 20705, USA 2 - Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, 27695, USA Corresponding authors: subodh.k.srivastava@usda.gov & Gloria.Abad@usda.gov

• Resource Title: Genome & Annotation Data: Genome Resources for the Ex-type of Phytophthora citricola, and well-authenticated isolates of P. hibernalis, P. nicotianae and P. syringae .

  File Name: Genome and annotation data.zip

  Resource Description: The Canu assembled genome was trained the prediction model using a previously sequenced species Phytophthora capsici as a model organism for AUGUSTUS software described in paper under review PHYTO-04-21-0167-A.