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Genomes & Annotations Data: Genome Resources for the Ex-type of Phytophthora citricola, and well-authenticated isolates of P. hibernalis, P. nicotianae and P. syringae

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posted on 2024-02-16, 17:24 authored by Subodh SrivastavaSubodh Srivastava, Z. Gloria Abad
<p><em>Phytophthora</em> is one of the most important genera of plant pathogens. In order to build robust molecular identification systems, it is very important to have information from well-authenticated specimens and in preference the Ex-type specimens. The reference genomes of well-authenticated specimens form a critical foundation for genetics, biological research, and diagnostic applications. In this study, we describe four draft <em>Phytophthora</em> genomes resources for the Ex-type of <em>P. citricola</em> BL34 (P0716 WPC) (118 contigs for 50 Mb), and well-authenticated specimens of <em>P. syringae</em> BL57G (P10330 WPC) (591 contigs for 75 Mb), <em>P. hibernalis</em> BL41G (P3822 WPC) (404 contigs for 84 Mb), and <em>P. nicotianae</em> BL162 (P6303 WPC) (3984 contigs for 108 Mb) generated with MinION long-read High-Throughput Sequencing (HTS) technology (Oxford Nanopore Technologies, ONT). Using these draft reads we assembled high coverage genomes of <em>P. citricola</em> with 291X coverage and 16,662 annotated genes; <em>P. nicotianae</em> with 205X coverage and 29,271 annotated genes; <em>P. syringae</em> with 76X coverage and 23,331 annotated genes, and <em>P. hibernalis</em> with 42X coverage and 21,762 annotated genes. We anticipate that these draft genomes will be helpful for various basic and applied research including diagnostics to protect US agriculture.</p> <p>Subodh K. Srivastava (1, 2), Leandra M. Knight (1, 2), Mark Nakhla (1) and Z. Gloria Abad (1) 1 - USDA APHIS PPQ, S&T, Beltsville Laboratory, Beltsville, MD, 20705, USA 2 - Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, 27695, USA Corresponding authors: subodh.k.srivastava@usda.gov & Gloria.Abad@usda.gov </p> <div><br>Resources in this dataset:</div><br><ul><li><p>Resource Title: Genome & Annotation Data: Genome Resources for the Ex-type of Phytophthora citricola, and well-authenticated isolates of P. hibernalis, P. nicotianae and P. syringae .</p> <p>File Name: Genome and annotation data.zip</p><p>Resource Description: The Canu assembled genome was trained the prediction model using a previously sequenced species Phytophthora capsici as a model organism for AUGUSTUS software described in paper under review PHYTO-04-21-0167-A.</p></li></ul>

Funding

USDA-APHIS: Farm Bill Section 10007

History

Data contact name

Srivastava, Subodh K.

Publisher

Ag Data Commons

Temporal Extent Start Date

2018-01-01

Temporal Extent End Date

2020-12-31

Theme

  • Not specified

Geographic Coverage

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ISO Topic Category

  • biota
  • environment

National Agricultural Library Thesaurus terms

plant pathogens; Phytophthora citricola; Phytophthora syringae; Phytophthora nicotianae; high-throughput nucleotide sequencing; genes; applied research; diagnostic techniques; United States

OMB Bureau Code

  • 005:32 - Animal and Plant Health Inspection Service

Primary article PubAg Handle

Pending citation

  • No

Public Access Level

  • Public

Preferred dataset citation

Srivastava, Subodh Kumar; Abad, Z. Gloria (2021). Genomes & Annotations Data: Genome Resources for the Ex-type of Phytophthora citricola, and well-authenticated isolates of P. hibernalis, P. nicotianae and P. syringae. Ag Data Commons. https://doi.org/10.15482/USDA.ADC/1522404

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