Genome resources of two pathotypes of the potato cyst nematode Globodera rostochiensis from New York

The potato cyst nematode, Globodera rostochiensis, is a regulated pest posing a serious threat to potato production worldwide. Although the endemic pathotype (Ro1) of G. rostochiensis has been confined to New York State for several decades as a result of quarantine regulations and management with resistant potato cultivars, a virulent pathotype, Ro2, has emerged, for which control measures are scarce. The ability to detect Ro2 early in fields is necessary to sustain the success of G. rostochiensis quarantine in the US. Here, we report the structural and comparative analysis of draft genome sequences of multiple single-cyst-derived Ro1 and Ro2 lines, propagated from original field populations. Sequence variations identified are good targets for developing molecular diagnostic tools for differentiating G. rostochiensis pathotypes in NY.